A Method to Measure Myocardial Calcium Handling in Adult Drosophila
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چکیده
منابع مشابه
A method to measure myocardial calcium handling in adult Drosophila.
RATIONALE Normal cardiac physiology requires highly regulated cytosolic Ca(2+) concentrations and abnormalities in Ca(2+) handling are associated with heart failure. The majority of approaches to identifying the components that regulate intracellular Ca(2+) dynamics rely on cells in culture, mouse models, and human samples. However, a genetically robust system for unbiased screens of mutations ...
متن کاملNew Methods in Cardiovascular Biology A Method to Measure Myocardial Calcium Handling in Adult Drosophila
Rationale: Normal cardiac physiology requires highly regulated cytosolic Ca concentrations and abnormalities in Ca handling are associated with heart failure. The majority of approaches to identifying the components that regulate intracellular Ca dynamics rely on cells in culture, mouse models, and human samples. However, a genetically robust system for unbiased screens of mutations that affect...
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Maternal protein restriction (MPR) during pregnancy is associated with increased cardiovascular risk in the offspring in adulthood. In this study we evaluated the cardiac function of young male rats born from mothers subjected to MPR during pregnancy, focusing on the myocardial mechanics and calcium-handling proteins. After weaning, rats received normal diet until 3 mo old, when the following p...
متن کاملCalcium-regulated renal calcium handling in healthy men: relationship to sodium handling.
Several studies have shown an increase in urinary calcium excretion in response to a calcium load. However, because of the inverse changes in PTH levels with a calcium load, the effect of changes in serum calcium per se on its own excretion is unclear in humans. In this study we used a PTH clamp protocol to further characterize calcium-regulated renal calcium and magnesium handling and the rela...
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OBJECTIVE We examined the functional consequences of expressing adult rabbit fast skeletal sarcoplasmic reticulum (SR) Ca(2+)-ATPase (SERCA1a) in isolated adult rat ventricular myocytes. METHODS Myocytes were infected with a recombinant adenovirus harboring SERCA1a. Then 2 days after myocyte infection, protein expression was estimated using Western blot and SDS-PAGE analysis. We also measured...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 2011
ISSN: 0009-7330,1524-4571
DOI: 10.1161/circresaha.110.238105